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Subgingival bacterial community profiles in HIV ‐infected Brazilian adults with chronic periodontitis
Author(s) -
Ferreira D. C.,
Gonçalves L. S.,
Siqueira J. F.,
Carmo F. L.,
Santos H. F.,
Feres M.,
Figueiredo L. C.,
Soares G. M.,
Rosado A. S.,
Santos K. R. N.,
Colombo A. P. V.
Publication year - 2016
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12287
Subject(s) - chronic periodontitis , periodontitis , temperature gradient gel electrophoresis , microbiology and biotechnology , biofilm , biology , bleeding on probing , human immunodeficiency virus (hiv) , 16s ribosomal rna , bacteria , dentistry , medicine , immunology , genetics
Background and Objective To compare the subgingival microbial diversity between non‐ HIV ‐infected and HIV ‐infected individuals with chronic periodontitis using denaturing gradient gel electrophoresis ( DGGE ). Material and Methods Thirty‐two patients were selected: 11 were HIV ‐infected and 21 were non‐ HIV ‐infected, and all had chronic periodontitis. Periodontal measurements included probing depth, clinical attachment level, visible supragingival biofilm and bleeding on probing. Subgingival biofilm samples were collected from periodontal sites (50% with probing depth ≤ 4 mm and 50% with probing depth ≥ 5 mm) and whole‐genomic‐amplified DNA was obtained. The DNA samples were subjected to amplification of a 16S rRNA gene fragment using universal bacterial primers, followed by DGGE analysis of the amplified gene sequences. Results The non‐ HIV ‐infected group presented higher mean full‐mouth visible supragingival biofilm (p = 0.004), bleeding on probing (p = 0.006), probing depth (p < 0.001) and clinical attachment level (p = 0.001) in comparison with the HIV ‐infected group. DGGE analysis revealed 81 distinct bands from all 33 individuals. Banding profiles revealed a higher diversity of the bacterial communities in the subgingival biofilm of HIV ‐infected patients with chronic periodontitis. Moreover, cluster and principal component analyses demonstrated that the bacterial community profiles differed between these two conditions. High interindividual and intra‐individual variability in banding profiles were observed for both groups. Conclusion HIV ‐infected patients with chronic periodontitis present greater subgingival microbial diversity. In addition, the bacterial communities associated with HIV ‐infected and non‐ HIV ‐infected individuals are different in structure.