z-logo
Premium
Tumor necrosis factor‐α enhances RANKL expression in gingival epithelial cells via protein kinase A signaling
Author(s) -
Fujihara R.,
Usui M.,
Yamamoto G.,
Nishii K.,
Tsukamoto Y.,
Okamatsu Y.,
Sato T.,
Asou Y.,
Nakashima K.,
Yamamoto M.
Publication year - 2014
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12131
Subject(s) - rankl , osteoprotegerin , periodontal fiber , tumor necrosis factor alpha , cementum , osteoclast , periodontitis , receptor , chemistry , cancer research , medicine , activator (genetics) , pathology , dentistry , dentin
Objective and Background Periodontitis is an inflammatory disorder of the supporting tissue of teeth, which is composed of gingival soft tissue, cementum covering the tooth root, alveolar bone and periodontal ligament. The receptor activator of nuclear factor kappa B ligand ( RANKL ) is known to be an essential factor for osteoclastogenesis. Recent clinical studies indicate that levels of RANKL in the gingival crevicular fluid are increased while levels of its decoy receptor, osteoprotegerin ( OPG ), are decreased in patients with periodontitis. Although the gingival sulcus is composed of gingival tissue, RANKL and OPG expression in gingival epithelial cells is not fully understood. The aim of this study is to investigate the expression of RANKL and OPG in gingival tissue and which factors regulate RANKL expression in gingival epithelial cells. Material and Methods Reverse transcriptase polymerase chain reaction analysis, western blotting and immunohistochemistry were performed to confirm RANKL and OPG expression in gingival epithelial cells ( GEC s) and in gingival tissue. Immunostaining was also examined to confirm tumor necrosis factor ( TNF )‐α and TNF receptor type 1 ( TNFR 1) expression in gingival tissue. Ca9‐22 cells, a human gingival epithelial cell line and human primary GEC s were treated with TNF ‐α. Ca9‐22 cells were treated by antibodies against TNF receptors, an inhibitor and an activator of protein kinase A ( PKA ) signaling and inhibitors of p38, Erk and NF ‐κB signaling to examine TNF ‐α‐ RANKL signaling pathways. Results RANKL mRNA and protein were expressed in GECs. Immunohistochemistry also showed RANKL expression in gingival tissue. On the other hand, the reverse transcriptase polymerase chain reaction and immunohistochemistry assay showed that GECs did not express OPG. In addition, TNF‐α and TNFR1 proteins were expressed in junctional epithelium. TNF‐α increased RANKL expression in GECs. TNF‐α‐induced RANKL expression was inhibited by an antibody against TNFR1 and an inhibitor of PKA signaling. Surprisingly, forskolin, a PKA activator, increased TNF‐α‐induced RANKL expression. Conclusion RANKL , TNF and TNFR 1 were coexpressed in junctional epithelium of gingival tissue. TNF ‐α induced RANKL expression via TNFR 1 and PKA signaling in GEC s of junctional epithelium.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here