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Boric acid inhibits alveolar bone loss in rats by affecting RANKL and osteoprotegerin expression
Author(s) -
Sağlam M.,
Hatipoğlu M.,
Köseoğlu S.,
Esen H. H.,
Kelebek S.
Publication year - 2014
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12126
Subject(s) - osteoprotegerin , dental alveolus , rankl , molar , ligature , periodontitis , medicine , endocrinology , boric acid , chemistry , dentistry , receptor , activator (genetics) , organic chemistry
Background and Objective The goal of the present study was to evaluate the effects of systemic boric acid on the levels of expression of RANKL and osteoprotegerin ( OPG ) and on histopathologic and histometric changes in a rat periodontitis model. Material and Methods Twenty‐four Wistar rats were divided into three groups of eight animals each: nonligated ( NL ); ligature only ( LO ); and ligature plus treatment with boric acid ( BA ) (3 mg/kg per day for 11 d). A 4/0 silk suture was placed in a subgingival position around the mandibular right first molars; after 11 d the rats were killed, and alveolar bone loss in the first molars was histometrically determined. Periodontal tissues were examined histopathologically to assess the differences among the study groups. RANKL and OPG were detected immunohistochemically. Results Alveolar bone loss was significantly higher in the LO group than in the BA and NL groups ( p < 0.05). The number of inflammatory infiltrate and osteoclasts in the LO group was significantly higher than that in the NL and BA groups ( p < 0.05). The numbers of osteoblasts in LO and BA groups were significantly higher compared with NL group ( p < 0.05). There were significantly more RANKL‐positive cells in the LO group than in the BA and NL groups ( p < 0.05). There was a higher number of OPG ‐positive cells in the BA group than in the LO and NL groups ( p < 0.05). Conclusion The present study shows that systemic administration of boric acid may reduce alveolar bone loss by affecting the RANKL / OPG balance in periodontal disease in rats.