Premium
Follicular dendritic cell‐secreted protein is decreased in experimental periodontitis concurrently with the increase of interleukin‐17 expression and the Rankl / O pg mRNA ratio
Author(s) -
Takahashi S.,
Fukuda M.,
Mitani A.,
Fujimura T.,
Iwamura Y.,
Sato S.,
Kubo T.,
Sugita Y.,
Maeda H.,
Shinomura T.,
Noguchi T.
Publication year - 2014
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12118
Subject(s) - rankl , periodontitis , osteoprotegerin , dental alveolus , medicine , bone resorption , resorption , inflammation , interleukin , follicular phase , dendritic cell , immunology , andrology , endocrinology , cytokine , dentistry , receptor , immune system , activator (genetics)
Background and Objective T‐helper type 17 ( T h17) cells produce interleukin‐17 ( IL ‐17) and help to protect against inflammation and infection in periodontal disease. Furthermore, while follicular dendritic cell‐secreted protein ( FDC ‐ SP ) may be involved in the inflammation of periodontal tissue, the biological role of FDP ‐ SP in periodontal disease is still unknown. The purpose of the present study was to clarify the expression of IL ‐17 and FDC ‐ SP in experimental periodontitis in rats. Material and Methods Seven‐week‐old male Wistar rats were divided into baseline control, sham and test groups. Experimental periodontitis was induced by placing a ligature in the mesiopalatal area, and untreated rats served as a baseline control group. Morphological changes in alveolar bone were investigated 7, 14 and 28 d after treatment. Expression of the Rankl , osteoprotegerin ( Opg ) and Il 17 genes was analyzed 5 and 7 d after the induction of experimental periodontitis. Results Alveolar bone resorption progressed in the test group for 7 d, but not thereafter. At 5 d after the induction of periodontitis, the Rankl / Opg mRNA ratio and the expression of IL ‐17 in the test group were significantly increased compared with the respective values in the baseline control group; however, there were no significant differences between the test and control groups at 7 d. The expression of FDC ‐ SP was significantly decreased in the test group compared with the baseline control group at 5 and 7 d after the induction of periodontitis, and this value had returned to normal levels at 14 and 28 d. Conclusion These results suggest that both IL ‐17 and FDC ‐ SP could be involved in the inflammatory response, and FDC ‐ SP in the junctional epithelium might play an important role in the T h17 cell‐related immune response.