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Expression of extracellular matrix metalloproteinase inducer glycosylation and c aveolin‐1 in healthy and inflamed human gingiva
Author(s) -
Wang J.,
Yang D.,
Li C.,
Shang S.,
Xiang J.
Publication year - 2014
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12095
Subject(s) - basigin , matrix metalloproteinase , chemistry , periodontitis , metalloproteinase , extracellular matrix , glycosylation , western blot , immunofluorescence , microbiology and biotechnology , immunology , medicine , antibody , biology , biochemistry , gene
Background and Objective Glycosylated extracellular matrix metalloproteinase inducer ( EMMPRIN ) is specifically associated with c aveolin‐1 and influences its ability to induce matrix metalloproteinases ( MMP s) production. This study investigated EMMPRIN glycosylation and c aveolin‐1 expression in healthy and inflamed human gingival tissues, analyzed the relationship between EMMPRIN glycosylation and c aveolin‐1 expression, and assessed how this interaction influenced MMP ‐1 production. Material and Methods Gingival tissues were collected from 10 healthy subjects and 15 chronic periodontitis ( chronic periodontitis ) subjects. EMMPRIN , caveolin‐1 and MMP ‐1 expressions were analyzed by immunohistochemistry. EMMPRIN and c aveolin‐1 co‐localization was detected by immunofluorescence. EMMPRIN glycosylation, c aveolin‐1, active MMP ‐1 and pro MMP ‐1 expression was assessed by Western blot. Results EMMPRIN was expressed in gingival epithelial cells, inflammatory cells, endothelial and fibroblast‐like cells. Strong c aveolin‐1 immunoreactivity was detected in gingival epithelial and endothelial cells. Double immunofluorescence studies revealed EMMPRIN and c aveolin‐1 co‐localization in gingival epithelium, endothelial and fibroblast‐like cells. Compared with healthy subjects, the chronic periodontitis group had increased high‐glycoform EMMPRIN ( HG ‐ EMMPRIN ) and active MMP ‐1 expression ( p < 0.05). Active MMP ‐1 and pro MMP ‐1 protein levels were positively correlated with HG ‐ EMMPRIN levels ( p < 0.05). Conclusion EMMPRIN and c aveolin‐1 colocalize in periodontal tissues. The increased active MMP ‐1 and pro MMP ‐1 production may be associated with elevated HG ‐ EMMPRIN levels.