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S ynergistetes cluster A in saliva is associated with periodontitis
Author(s) -
Belibasakis G. N.,
Öztürk V.Ö.,
Emingil G.,
Bostanci N.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12061
Subject(s) - periodontitis , saliva , chronic periodontitis , microbiology and biotechnology , medicine , significant difference , aggressive periodontitis , dentistry , biology , gastroenterology , immunology
Background and Objectives S ynergistetes is a novel bacterial phylum consisting of gram‐negative anaerobes. Increasing lines of evidence demonstrate that this phylum is associated with periodontal diseases. This study aimed to compare the presence and levels of S ynergistetes clusters A and B , in saliva of patients with chronic periodontitis ( CP ), generalized aggressive periodontitis ( G ‐ A g P ) and non‐periodontitis subjects, and investigate their correlation with clinical parameters. Material and Methods Saliva was collected from patients with CP ( n = 20), G ‐ A g P ( n = 21) and non‐periodontitis subjects ( n = 18). Full mouth clinical periodontal measurements were recorded. The numbers of S ynergistetes cluster A and cluster B or the associated species J onquetella anthropi were quantified by fluorescent in situ hybridization and microscopy. Results S ynergistetes cluster A bacteria were detected more frequently, and at higher numbers and proportions in the two periodontitis groups, than the non‐periodontitis control group. The prevalence was 27.7% in the control group, 85% in CP and 86% in G ‐ A g P . Compared to the control group, the numbers were significantly higher by 12.5‐fold in CP and 26.5‐fold in G ‐ A g P , whereas the difference between the two forms of periodontitis was not statistically significant. Within the total bacterial population, the proportion of this cluster was increased in CP and G ‐ A g P compared to the control group, with the difference between the two forms of periodontitis being also significant. There was a positive correlation between the levels of S ynergistetes cluster A in saliva and all full mouth clinical periodontal parameters. Nevertheless, S ynergistetes cluster B bacteria and J . anthropi species were detected infrequently and at low levels in all the three subject groups. Conclusion S ynergistetes cluster A , but not cluster B , bacteria are found at higher prevalence, numbers and proportions in saliva from patients with periodontitis, than non‐periodontitis subjects. These findings support the association of this cluster with periodontitis.