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Altered relationship between MMP ‐8 and TIMP ‐2 in gingival crevicular fluid in adolescents with Down's syndrome
Author(s) -
Tsilingaridis G.,
YucelLindberg T.,
Modéer T.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12038
Subject(s) - gingivitis , matrix metalloproteinase , periodontitis , medicine , endocrinology , gastroenterology , chemistry , dentistry
Background and Objective Periodontitis is more frequently found in subjects with Down's syndrome. The aim was to investigate whether the relationship between MMP s and TIMP s) in the gingival crevicular fluid of subjects with Down's syndrome is altered compared with controls. Material and Methods Twenty‐one adolescents with Down's syndrome and gingivitis ( DS ‐G), 12 subjects with Down's syndrome and periodontitis ( DS ‐P), 26 controls with gingivitis ( HC ‐G) and eight controls with periodontitis ( HC ‐P) were clinically examined. All patients were between 11 and 20 years of age. Gingival crevicular fluid was collected from each subject and the concentrations of MMP s (2, 3, 8, 9 and 13) and TIMP s (1, 2 and 3) (expressed as pg/μL adjusted for volume of gingival crevicular fluid) were determined using multianalyte kits from R&D Systems. Results The concentrations of MMP ‐2, MMP ‐3, MMP ‐8, MMP ‐9 and TIMP ‐2 in gingival crevicular fluid were significantly higher ( p < 0.005) in the DS ‐G group compared with the HC ‐G group. The correlation coefficient between MMP ‐8 and TIMP ‐2 differed significantly ( p = 0.006) between the DS ‐G group and the HC ‐G group. On the contrary, the correlation coefficients between MMP s and TIMP s did not differ significantly between the DS ‐P group and the HC ‐P group. However, the DS ‐P group exhibited a significantly lower concentration of TIMP ‐2 in the gingival crevicular fluid compared with the HC ‐P group. Conclusion Down's syndrome subjects with gingivitis exhibit higher concentrations of MMP s in gingival crevicular fluid with an altered relationship between MMP ‐8 and TIMP ‐2, which might impair the periodontal tissue turnover.