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Evaluation of three selective media for isolation of A ggregatibacter actinomycetemcomitans
Author(s) -
Rurenga P.,
Raangs E.,
Singadji Z.,
WekemaMulder G.,
Veloo A. C. M.,
Winkelhoff A. J.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12037
Subject(s) - brain heart infusion , aggregatibacter actinomycetemcomitans , microbiology and biotechnology , colony forming unit , agar , isolation (microbiology) , vancomycin , agar plate , chocolate agar , pathogen , biology , bacitracin , medicine , bacteria , dentistry , antibiotics , periodontitis , staphylococcus aureus , porphyromonas gingivalis , genetics
Background and Objective Aggregatibacter actinomycetemcomitans is a pathogen in oral and nonoral infections. Detection and quantification of this pathogen can be performed using selective culture techniques. The aim of this study was to establish the efficacy of two known selective media in their ability to select and support the growth of A . actinomycetemcomitans . Material and Methods Trypticase soy bacitracin vancomycin ( TSBV ) medium and brain–heart infusion agar with vancomycin ( D entaid‐1), as well as a modified D entaid‐1 medium (in which the brain–heart infusion agar was substituted with brain–heart infusion broth), were compared. Two‐hundred and eighteen clinical samples were used to establish the recovery rate, the number of colony‐forming units (CFUs) of A . actinomycetemcomitans as well as the total number of CFUs on the three different types of medium. In addition, the numbers of gram‐negative aerobic rods and yeasts were determined. Results Both types of D entaid‐1 medium showed a higher recovery of A . actinomycetemcomitans compared with TSBV . However, these differences did not reach statistical significance. The total number of CFUs of A . actinomycetemcomitans recovered was significantly higher on D entaid‐1 compared with TSBV ( p = 0.029). The mean number of gram‐negative aerobic rods recovered was statistically higher on both types of D entaid‐1 medium in comparison with TSBV . Low numbers of yeasts were recovered occasionally on all test plates. Conclusion Dentaid‐1 is a low‐cost effective alternative to TSBV for the isolation and growth of A . actinomycetemcomitans from clinical samples, such as dental plaque, which contain a complex microflora.