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Expression profile of macrophage migration‐inhibitory factor in human gingiva and reconstituted human gingival epithelia stimulated by Porphyromonas gingivalis lipopolysaccharide
Author(s) -
Li X.,
Lan H. Y.,
Huang X. R.,
Zhang C.,
Jin L. J.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12035
Subject(s) - porphyromonas gingivalis , macrophage migration inhibitory factor , lipopolysaccharide , periodontitis , connective tissue , inflammation , macrophage , immunohistochemistry , pathogenesis , chemistry , biology , immunology , microbiology and biotechnology , in vitro , pathology , medicine , cytokine , biochemistry
Background and Objective Macrophage migration‐inhibitory factor ( MIF ) plays crucial roles in the recruitment and activation of macrophages as well as in helping to kill bacteria. This study investigated the expression profile of MIF in human gingiva under different periodontal conditions and its expression patterns induced by P orphyromonas gingivalis lipopolysaccharide ( LPS ) in gingival epithelia. Material and Methods Gingival tissue samples were collected from deep pockets and clinically healthy sites of 22 nonsmoking subjects with chronic periodontitis. The expression of MIF m RNA and protein was evaluated using real‐time PCR and immunohistochemistry, respectively. The in vitro study analyzed the effects of P . gingivalis LPS on the expression of MIF in a reconstituted human gingival epithelia ( RHGE ) model. Results In gingival epithelia, MIF protein was diffusely expressed from the basal layer to the granular and spinous layers; whereas, in the underlying connective tissues, MIF was observed around the dilated blood vessels in the deep‐pocket tissues. A significantly lower level of expression of MIF m RNA and an increased level of expression of MIF protein were found in deep‐pocket tissues compared with clinically healthy tissues. Expression of MIF m RNA in the RHGE model was significantly down‐regulated by P . gingivalis LPS . Conclusion The present study suggests that MIF expression may be related to periodontal conditions and that its expression profile could be modulated by P . gingivalis LPS . MIF may play a role in periodontal pathogenesis.