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Excessive W nt/β‐catenin signaling disturbs tooth‐root formation
Author(s) -
Bae C. H.,
Lee J. Y.,
Kim T. H.,
Baek J. A.,
Lee J. C.,
Yang X.,
Taketo M. M.,
Jiang R.,
Cho E. S.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12018
Subject(s) - dentin sialophosphoprotein , dentin , odontoblast , cementum , cementoblast , molar , chemistry , microbiology and biotechnology , wnt signaling pathway , dental cementum , dental papilla , mutant , root canal , dentistry , signal transduction , biology , medicine , biochemistry , gene
Background and Objective Wingless‐type MMTV integration site family (W nt)/β‐catenin signaling plays an essential role in cellular differentiation and matrix formation during skeletal development. However, little is known about its role in tooth‐root formation. In a previous study, we found excessive formation of dentin and cementum in mice with constitutive β‐catenin stabilization in the dental mesenchyme. In the present study we analyzed the molar roots of these mice to investigate the role of W nt/β‐catenin signaling in root formation in more detail. Material and Methods We generated OC ‐ C re: C atnb +/lox(ex3) mice by intercrossing C atnb +/lox(ex3) and OC ‐ C re mice, and we analyzed their mandibular molars using radiography, histomorphometry and immunohistochemistry. Results OC ‐ C re: C atnb +/lox(ex3) mice showed impaired root formation. At the beginning of root formation in mutant molars, dental papilla cells did not show normal differentiation into odontoblasts; rather, they were prematurely differentiated and had a disorganized arrangement. Interestingly, SMAD family member 4 was upregulated in premature odontoblasts. In 4‐wk‐old mutant mice, molar roots were about half the length of those in their wild‐type littermates. In contrast to excessively formed dentin in crown, root dentin was thin and hypomineralized in mutant mice. B iglycan and dentin sialophosphoprotein were downregulated in root dentin of mutant mice, whereas dentin matrix protein 1 and D ickkopf‐related protein 1 were upregulated. Additionally, ectonucleotide pyrophosphatase/phosphodiesterase 1 was significantly downregulated in the cementoblasts of mutant molars. Finally, in the cementum of mutant mice, bone sialoprotein was downregulated but D ickkopf‐related protein 2 was upregulated. Conclusion These results suggest that temporospatial regulation of W nt/β‐catenin signaling plays an important role in cell differentiation and matrix formation during root and cementum formation.