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Effect of bone morphogenetic protein‐2, demineralized bone matrix and systemic parathyroid hormone (1‐34) on local bone formation in a rat calvaria critical‐size defect model
Author(s) -
Stancoven B. W.,
Lee J.,
Dixon D. R.,
McPherson J.C.,
Bisch F. C.,
Wikesjö U. M. E.,
Susin C.
Publication year - 2013
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12001
Subject(s) - calvaria , parathyroid hormone , bone healing , demineralized bone matrix , medicine , endocrinology , bone morphogenetic protein 2 , dbm , bone morphogenetic protein , bone formation , chemistry , surgery , calcium , in vitro , amplifier , biochemistry , cmos , electronic engineering , engineering , gene
Aim To determine the potential of recombinant human bone morphogenetic protein‐2 (rh BMP ‐2) soak‐loaded on to an absorbable collagen sponge ( ACS ) to induce local bone formation compared with the clinical reference demineralized bone matrix ( DBM ) and to investigate potential additive/synergistic effects of exogenous parathyroid hormone ( PTH ). Methods Critical‐size (8 mm), through‐through calvaria osteotomy defects in 160 adult male S prague– D awley rats were randomized to receive one of eight interventions: rh BMP ‐2/ ACS , DBM , ACS , or serve as controls (empty defects) combined or not with systemic PTH . Ten animals from each group were followed for 4 and 8 wks for radiographic and histometric analysis. Multivariable analysis was used to assess the effect of experimental intervention and healing time on local bone formation. Results In the multivariable analysis, rh BMP ‐2/ ACS exhibited significantly greater histologic bone formation than control (β ± SE : 54.76 ± 5.85, p < 0.001) and ACS (β ± SE : 9.14 ± 3.31, p = 0.007) whereas DBM showed significantly less bone formation than control (β ± SE : −32.32 ± 8.23, p < 0.001). Overall, PTH did not show a significant effect on bone formation (β ± SE : 2.72 ± 6.91, p = 0.70). No significant differences in histological defect closure were observed between 4 and 8 wks for all but the control group without PTH . Conclusion rh BMP ‐2/ ACS significantly stimulates local bone formation whereas bone formation appears significantly limited by DBM . Systemic application of PTH provided no discernible additive/synergistic effects on local bone formation.