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Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics
Author(s) -
Mazumdar Nilanjana,
Novis Phil M.,
Visnovsky Gabriel,
Gostomski Peter
Publication year - 2019
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12889
Subject(s) - photobioreactor , biology , aeration , growth rate , botany , strain (injury) , kinetics , airlift , biomass (ecology) , bioreactor , food science , ecology , physics , geometry , mathematics , anatomy , quantum mechanics
The present study investigated the effect of different culture conditions on the vegetative growth of a new species, Haematococcus alpinus (strain LCR ‐ CC ‐261f) using airlift photobioreactors. The influence of culture medium, aeration rates, CO 2 concentration in air‐gas mixture, temperature, light intensities, and wavelengths were investigated to achieve sustainable high cell density cultures. Growth parameters were determined by fitting the data to a form of the logistic equation that included a lag phase. The shear‐sensitive vegetative cells favored lower aeration rates in the photobioreactors. MLA medium increased to 40 mM nitrate produced high density cultures. Temperatures between 12°C and 18°C, 3% (v/v) CO 2 concentration and a narrow photon flux density ranging between 37 and 48 μmol photons · m −2 · s −1 were best suited for growth. The wavelength of the light source also impacted growth and a high cell density of 9.6 × 10 5 cells · mL −1 was achieved using a mixture of red and blue compared to warm white, red, or blue LED s.