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Different fermentation strategies by Schizochytrium mangrovei strain pq6 to produce feedstock for exploitation of squalene and omega‐3 fatty acids
Author(s) -
Hoang Lan Anh Thi,
Nguyen Ha Cam,
Le Thom Thi,
Hoang Thi Huong Quynh,
Pham Van Nhat,
Hoang Minh Hien Thi,
Ngo Hoai Thu Thi,
Hong Dang Diem
Publication year - 2018
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12757
Subject(s) - food science , squalene , fermentation , biology , biomass (ecology) , docosahexaenoic acid , fatty acid , biochemistry , polyunsaturated fatty acid , agronomy
Schizochytrium mangrovei strain PQ 6 was investigated for coproduction of docosahexaenoic acid (C22: 6ω‐3, DHA ) and squalene using a 30‐L bioreactor with a working volume of 15 L under various batch and fed‐batch fermentation process regimes. The fed‐batch process was a more efficient cultivation strategy for achieving higher biomass production rich in DHA and squalene. The final biomass, total lipid, unsaponifiable lipid content, and DHA productivity were 105.25 g · L −1 , 43.40% of dry cell weight, 8.58% total lipid, and 61.66 mg · g −1 · L −1 , respectively, after a 96 h fed‐batch fermentation. The squalene content was highest at 48 h after feeding glucose (98.07 mg · g −1 of lipid). Differences in lipid accumulation during fermentation were correlated with changes in ultrastructure using transmission electron microscopy and Nile Red staining of cells. The results may be of relevance to industrial‐scale coproduction of DHA and squalene in heterotrophic marine microalgae such as Schizochytrium .