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Kinetic flux profiling dissects nitrogen utilization pathways in the oleaginous green alga Chlorella protothecoides
Author(s) -
Wu Chao,
Xiong Wei,
Dai Junbiao,
Wu Qingyu
Publication year - 2016
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12374
Subject(s) - biology , nitrogen assimilation , ammonium , glutamate dehydrogenase , glutamate synthase , glutamine synthetase , biochemistry , nitrogen , nitrogen cycle , metabolic pathway , assimilation (phonology) , glutamine , botany , metabolism , enzyme , glutamate receptor , chemistry , amino acid , receptor , linguistics , organic chemistry , philosophy
As a promising candidate for biodiesel production, the green alga Chlorella protothecoides can efficiently produce oleaginous biomass and the lipid biosynthesis is greatly influenced by the availability of nitrogen source and corresponding nitrogen assimilation pathways. Based on isotope‐assisted kinetic flux profiling ( KFP ), the fluxes through the nitrogen utilization pathway were quantitatively analyzed. We found that autotrophic C. protothecoides cells absorbed ammonium mainly through glutamate dehydrogenase ( GDH ), and partially through glutamine synthetase ( GS ), which was the rate‐limiting enzyme of nitrogen assimilation process with rare metabolic activity of glutamine oxoglutarate aminotransferase ( GOGAT , also known as glutamate synthase); whereas under heterotrophic conditions, the cells adapted to GS‐GOGAT cycle for nitrogen assimilation in which GS reaction rate was associated with GOGAT activity. The fact that C. protothecoides chooses the adenosine triphosphate‐free and less ammonium‐affinity GDH pathway, or alternatively the energy‐consuming GS ‐ GOGAT cycle with high ammonium affinity for nitrogen assimilation, highlights the metabolic adaptability of C. protothecoides exposed to altered nitrogen conditions.