Premium
Examination of the structures of several glycerolipids from marine macroalgae by NMR and GC ‐ MS
Author(s) -
Logvinov Stepan,
Gerasimenko Natalia,
Esipov Andrey,
Denisenko Vladimir A.
Publication year - 2015
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12338
Subject(s) - galactolipids , brown algae , algae , biology , galactolipid , glycolipid , biochemistry , nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , mass spectrometry , botany , stereochemistry , chromatography , chemistry , phospholipid , chloroplast , membrane , gene
Several classes of glycerolipids were isolated from the total lipids of the algae Saccharina cichorioides , Eualaria fistulosa , Fucus evanescens , Sargassum pallidum , Silvetia babingtonii (Ochrophyta, Phaeophyceae), Tichocarpus crinitus , and Neorhodomela larix (Rhodophyta, Florideophyceae). The structures of these lipids were examined by nuclear magnetic resonance ( NMR ) spectroscopy, including 1D ( 1 H and 13 C) and 2D ( COSY , HSQC and HMBC ) experiments. All of the investigated algae included common galactolipids and sulfonoglycolipids as the major glycolipids. Minor glycolipids isolated from S. cichorioides , T. crinitus , and N. laris were identified as lyso‐galactolipids with a polar group consisted of the galactose. Comparison of the 1 H NMR data of minor nonpolar lipids isolated from the extracts of the brown algae S. pallidum and F. evanescens with the 1 H NMR data of other lipids allowed them to be identified as diacylglycerols. The structures of betaine lipids isolated from brown algae were confirmed by NMR for the first time. The fatty acid compositions of the isolated lipids were determined by gas chromatography‐mass spectrometry.