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Glycerol‐3‐phosphate metabolism plays a role in stress response in the red alga Pyropia haitanensis
Author(s) -
Lai XiaoJuan,
Yang Rui,
Luo QiJun,
Chen JuanJuan,
Chen HaiMin,
Yan XiaoJun
Publication year - 2015
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12276
Subject(s) - biology , dehydrogenase , biochemistry , metabolism , algae , glycerol 3 phosphate dehydrogenase , enzyme , microbiology and biotechnology , botany
Glycerol‐3‐phosphate (G3P) has been suggested as a novel regulator of plant defense signaling, however, its role in algal resistance remains largely unknown. The glycerol kinase (also designated as NHO 1 ) and NAD ‐dependent G3P dehydrogenase ( GPDH ) are two key enzymes involved in the G3P biosynthesis. In our study, we cloned the full‐length cDNA of NHO 1 ( NHO 1 Ph ) and GPDH ( GPDH Ph ) from the red alga Pyropia haitanensis (denoted as NHO 1 Ph and GPDH Ph ) and examined their expression level under flagellin peptide 22 (flg22) stimulation or heat stress. We also measured the level of G3P and floridoside (a downstream product of G3P in P. haitanensis ) under flg22 stimulation or heat stress. Both NHO 1 Ph and GPDH Ph shared high sequence identity and structural conservation with their orthologs from different species, especially from red algae. Phylogenetic analysis showed that NHO 1 s and GPDH s from red algae were closely related to those from animals. Under flg22 stimulation or heat stress, the expression levels of NHO 1 Ph and GPDH Ph were up‐regulated, G3P levels increased, and the contents of floridoside decreased. But the floridoside level increased in the recovery period after heat stress. Taken together, we found that G3P metabolism was associated with the flg22‐induced defense response and heat stress response in P. haitanensis , indicating the general conservation of defense response in angiosperms and algae. Furthermore, floridoside might also participate in the stress resistance of P. haitanensis .