Premium
Phylogeny of N orth A merican T olypella ( C harophyceae, C harophyta) based on plastid DNA sequences with a description of T olypella ramosissima sp. nov.
Author(s) -
Pérez William,
Hall John D.,
McCourt Richard M.,
Karol Kenneth G.
Publication year - 2014
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12219
Subject(s) - biology , monophyly , paraphyly , phylogenetic tree , phylogenetics , tribe , molecular phylogenetics , clade , genus , botany , zoology , genetics , gene , sociology , anthropology
C haraceae ( C harophyceae, C harophyta) contains two tribes with six genera: tribe C hareae with four genera and tribe N itelleae, which includes T olypella and N itella . This paper uses molecular and morphological data to elucidate the phylogeny of T olypella species in N orth A merica. In the most comprehensive taxonomic treatment of C haraceae, 16 T olypella species worldwide were subsumed into two species, T . intricata and T . nidifica , in two sections, R othia and T olypella respectively. It was further suggested that T olypella might be a derived group within N itella . In this investigation into species diversity and relationships in N orth A merican T olypella , sequence data from the plastid genes atp B, psb C, and rbc L were assembled for a broad range of charophycean and land plant taxa. Molecular data were used in conjunction with morphology to test monophyly of the genus and species within it. Phylogenetic analyses of the sequence data showed that C haraceae is monophyletic but that N itelleae is paraphyletic with T olypella sister to a monophyletic N itella + C hareae. The results also supported the monophyly of T olypella and the sections R othia and T olypella . Morphologically defined species were supported as clades with little or no DNA sequence differences. In addition, molecular data revealed several lineages and a new species ( T . ramosissima sp. nov.), which suggests greater species diversity in T olypella than previously recognized.