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Phylogenetic Reappraisal of the Genus Monomorphina (Euglenophyceae) Based on Molecular and Morphological Data
Author(s) -
Kim Jong Im,
Shin Woongghi,
Triemer Richard E.
Publication year - 2013
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1111/jpy.12018
Subject(s) - clade , biology , phylogenetic tree , sensu , genus , phylogenetics , molecular phylogenetics , evolutionary biology , botany , zoology , genetics , gene
A morphological and molecular examination of the genus M onomorphina was conducted on 46 strains isolated mainly from K orea. The strains were divided into two types based on morphological data: M onomorphina aenigmatica and M . pyrum ‐ like species. Phylogenetic analysis based on a combined data set of nuclear SSU and LSU and plastid SSU and LSU rDNA showed that the strains could be divided into eight clades: Clade A of M . aenigmatica , Clade B of the isolates ( M . pyropsis ) from M ichigan, USA , Clade C of M. pseudopyrum , Clade D of the isolates ( M . pyroria ) from Bremen, Germany, Clade E of M . soropyrum , Clade F of M . pyriformis , Clade G of M . parapyrum , and Clade H of M . pyrum . Six of these clades came from strains that would be considered M . pyrum sensu Kosmala et Zakryś, one of which could be recognized as a traditional species ( M. pyrum ) and five were designated as new species; each species had unique molecular signatures at nr SSU rDNA helix 17 and 17′ and spacer E23_14′‐E23_15. The species of Monomorphina had a wide range of genetic diversity with interspecies sequence similarity of 85.6%–97.1% and intraspecies similarity of 96.4%–99.9%. Our results suggested that genetic diversity found in the M . pyrum complex justifies the recognition of a minimum of eight species within this genus , based on specific molecular signatures and gene divergence of the nr SSU rDNA sequences.