The effects of eggshell calcium (Biomin H ® ) and its combinations with alfacalcidol (1α‐hydroxyvitamin D3) and menaquinone‐7 (vitamin K2) on ovariectomy‐induced bone loss in a rat model of osteoporosis | Zendy

Omelka Radoslav | Zendy; Martiniakova Monika | Zendy; Svik Karol | Zendy; Slovak Lukas | Zendy; Payer Juraj | Zendy; Oppenbergerova Ingrid | Zendy; Kovacova Veronika | Zendy; Babikova Martina | Zendy; SoltesovaPrnova Marta | Zendy

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The effects of eggshell calcium (Biomin H ® ) and its combinations with alfacalcidol (1α‐hydroxyvitamin D3) and menaquinone‐7 (vitamin K2) on ovariectomy‐induced bone loss in a rat model of osteoporosis

Author(s) -

Omelka Radoslav,

Martiniakova Monika,

Svik Karol,

Slovak Lukas,

Payer Juraj,

Oppenbergerova Ingrid,

Kovacova Veronika,

Babikova Martina,

SoltesovaPrnova Marta

Publication year - 2021

Publication title -

journal of animal physiology and animal nutrition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.651

H-Index - 56

eISSN - 1439-0396

pISSN - 0931-2439

DOI - 10.1111/jpn.13458

Subject(s) - ovariectomized rat , alfacalcidol , medicine , endocrinology , chemistry , calcium , vitamin d and neurology , bone mineral , deoxypyridinoline , eggshell , vitamin , osteoporosis , alkaline phosphatase , osteocalcin , estrogen , biology , biochemistry , enzyme , ecology

The purpose of this study was to investigate the impact of eggshell calcium (Biomin H ® dietary supplement) and its combinations with alfacalcidol (1α‐hydroxyvitamin D 3 ) and menaquinone‐7 (vitamin K 2 ) on ovariectomy‐induced bone loss in rats. Adult female rats ( n = 48) were divided into 6 groups of 8 individuals each: sham‐operated rats (SHAM); ovariectomized (OVX) rats untreated; OVX rats treated with Biomin H ® (BIO); OVX rats simultaneously receiving Biomin H ® , vitamin D 3 (BIO + D 3 ); OVX rats simultaneously treated with Biomin H ® , vitamin K 2 (BIO + K 2 ) and OVX rats treated with Biomin H ® , vitamin D 3 , vitamin K 2 (BIO + D 3 + K 2 ) during 8 weeks. Biochemical parameters, bone mineral density (BMD), bone mineral content (BMC) and femoral bone microstructure were determined. Plasma calcium and phosphate were increased in BIO + D 3 and BIO + D 3 + K 2 groups as compared to OVX. Alkaline phosphatase was elevated in OVX, BIO versus SHAM, BIO + D 3 + K2 groups. When compared to OVX group, decreased urine deoxypyridinoline was observed in all treated groups and femoral BMD, BMC were higher in BIO, BIO + D 3 , BIO + D 3 + K 2 groups. The BIO + K 2 rats had similar densitometrical values than OVX individuals. Microcomputed tomography revealed increased trabecular relative bone volume (due to an increase in trabecular number) in BIO + D 3 , BIO + D 3 + K 2 as compared to OVX. The higher relative bone volume in BIO + D 3 , BIO + D 3 + K 2 groups was also accompanied by an increase in bone surface. In the cortical bone, an enhanced periosteal bone apposition was identified in BIO, BIO + D 3 , BIO + K 2 , BIO + D 3 + K 2 groups. The rats from BIO + D 3 + K 2 group had a higher area of primary osteon's vascular canals. In BIO + D 3 , BIO + K 2 , BIO + D 3 + K 2 groups, an increased area of secondary osteons was determined in comparison with OVX. Our results indicate the beneficial effect of triple application of Biomin H ® , vitamin D 3 , vitamin K 2 , as well as simultaneous administration of Biomin H ® , vitamin D 3 on the inhibition of ovariectomy‐induced bone loss in a rat model of osteoporosis.

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