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Effects of dietary supplementation with l ‐arginine on the intestinal barrier function in finishing pigs with heat stress
Author(s) -
Yi Hongbo,
Xiong Yunxia,
Wu Qiwen,
Wang Mengzhu,
Liu Shuai,
Jiang Zongyong,
Wang Li
Publication year - 2020
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.13277
Subject(s) - occludin , barrier function , basal (medicine) , arginine , endocrinology , medicine , jejunum , chemistry , tight junction , heat stress , zoology , biology , amino acid , biochemistry , insulin , microbiology and biotechnology
Previous studies showed heat stress reduces body weight gain and feed intake associated with damaged intestinal barrier function, and l ‐arginine (L‐Arg) enhanced intestinal barrier function in young animals under stress. The aim of this study was to evaluate effects of L‐Arg on serum hormones, intestinal morphology, nutrients absorption and epithelial barrier functions in finishing pigs with heat stress. Forty‐eight finishing pigs (Landrace) were balanced for sex and then randomly assigned to six groups: TN group, thermal neutral (22°C, ~80% humidity) with a basal diet; HS group, heat stress (cyclical 35°C for 12 hr and 22°C for 12 hr, ~80% humidity) with a basal diet; PF group, thermal neutral (22°C, ~80% humidity) and pair‐fed with the HS; the TNA, HSA and PFA groups were the basal diet of TN group, HS group and PF group supplemented with 1% L‐Arg. Results showed that HS decreased ( p < .05) the thyroxine concentrations and increased ( p < .05) the insulin concentrations in serum compared with the TN group, but 1% L‐Arg had no significant effects on them. Both HS and PF significantly increased ( p < .05) the mRNA expression of cationic amino acid transporters (CAT1 and CAT2) and decreased the mRNA expression of solute carrier family 5 member 10 (SGLT1) in the jejunum compared with the TN group. Compared with the TN group, HS reduced the expression of tight junction (TJ) protein zonula occluden‐1 (ZO‐1) and occludin, but PF only decreased ZO‐1 expression in the jejunum. Results exhibited that dietary supplementation with 1% L‐Arg improved the intestinal villous height, the ratio of villous height to crypt depth, and the expression of occludin and porcine beta‐defensin 2 (pBD2) in the jejunum of intermittent heat‐treated finishing pigs. In conclusion, dietary supplementation with 1% L‐Arg could partly attenuate the intermittent heat‐induced damages of intestinal morphology and epithelial barrier functions in finishing pigs.