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Assessing the fate of recombinant plant DNA in rabbit’s tissues fed genetically modified cotton
Author(s) -
Shahid Ahmad Ali,
Salisu Ibrahim Bala,
Yaqoob Amina,
Rao Abdul Qayyum,
Ullah Inayat,
husnain Tayyab
Publication year - 2020
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.13243
Subject(s) - cry1ac , biology , genetically modified organism , exogenous dna , transgene , endogeny , gene , genetically modified crops , dna , recombinant dna , transfer dna , microbiology and biotechnology , genetics , biochemistry , agrobacterium tumefaciens
Various feeding studies have been conducted with the different species of animals to evaluate the possible transfer of transgenic DNA (tDNA) from genetically modified (GM) feed into the animal tissues. However, the conclusions drawn from most of such studies are sometimes controversial. Thus, in the present study, an attempt has been made to evaluate the fate of tDNA in rabbits raised on GM cotton‐based diet through PCR analysis of the DNA extracted specifically from blood, liver, kidney, heart and intestine (jejunum). A total of 48 rabbits were fed a mixed diet consisting variable proportions of transgenic cottonseeds meal (i.e. 0% w/w, 20% w/w, 30% w/w and 40% w/w) for 180 days. The presence of transgenic DNA fragments ( Cry1Ac, Cry2A and CP4 EPSPS ) or plant endogenous gene ( Sad1 ) was traced in those specific tissues and organs. The presence of β‐actin ( ACTB ) was also monitored as an internal control. Neither the transgenic fragments (459 bp of Cry1Ac gene, 167 bp of Cry2A gene and111 bp of CP4 EPSPS gene) nor cotton endogenous reference gene (155 bp of Sad1) could be detected in any of the DNA samples extracted from the rabbit's tissues in both control and transgenic groups. However, 155 bp fragment of the rabbit's reference gene ( ACTB ) was recovered in all the DNA samples extracted from rabbit tissues. The results obtained from this study revealed that both plant endogenous and transgenic DNA fragments have same fate in rabbit's tissues and were efficiently degraded in the gastrointestinal tract (GIT).