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Optimization of surfactin production from Bacillus subtilis in fermentation and its effects on Clostridium perfringens ‐induced necrotic enteritis and growth performance in broilers
Author(s) -
Cheng YeongHsiang,
Zhang Ning,
Han JinCheng,
Chang ChingWen,
Hsiao Felix ShihHsiang,
Yu YuHsiang
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12937
Subject(s) - clostridium perfringens , bacillus subtilis , surfactin , broiler , fermentation , food science , microbiology and biotechnology , biology , probiotic , clostridium , antimicrobial , bacteria , genetics
Bacillus species are commonly used as probiotics in the poultry feed industry for preventing infectious diseases and improving productivity by altering gastrointestinal microbiota. The growth parameters of Bacillus subtilis for surfactin production in fermentation and the benefits of surfactin on broiler chickens remain unclear. In this study, we examined the growth parameters of B. subtilis in fermentation and evaluated the effects of surfactin from B. subtilis ‐fermented products on Clostridium perfringens ‐induced necrotic enteritis and growth performance in broilers. Results showed that the highest viable biomass of B. subtilis was observed at 10% molasses and 2% yeast supplementation during fermentation. The 4‐ and 6‐day fermented B. subtilis products were heat‐, acid‐ and bile‐resistant. Furthermore, the 4‐day fermented B. subtilis products with the highest surfactin concentration showed the maximal antimicrobial activity against pathogens, including Escherichia coli , Staphylococcus aureus , Salmonella typhimurium and C. perfringens . Dietary B. subtilis ‐fermented product supplementation in broilers significantly improved intestinal morphology and necrotic lesions under C. perfringens challenge. Bacillus subtilis treatments could enhance broiler productivity, as well as promote bone quality and intestinal morphology. These results together indicate that B. subtilis ‐fermented products containing surfactin have potential for the development as feed additives and use as possible substitutes for antibiotics to treat C. perfringens in the poultry industry.