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Immunomodulatory effect of Artemisia argyi polysaccharide on peripheral blood leucocyte of broiler chickens
Author(s) -
Zhang P.,
Shi B.,
Li T.,
Xu Y.,
Jin X.,
Guo X.,
Yan S.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12895
Subject(s) - broiler , chemistry , nitric oxide , cytokine , receptor , nitric oxide synthase , in vitro , tumor necrosis factor alpha , antibody , medicine , immunology , endocrinology , biochemistry , food science
Summary This study was conducted to investigate the immunomodulatory effect of a water‐soluble polysaccharide extracted from Artemisia argyi ( AAP ) in vitro. The effect was assessed in peripheral blood leucocytes ( PBL s) of broilers, which were incubated with different AAP concentrations (0, 25, 50, 100, and 200 μg/ml) for 24 hr at 37°C in a 5% CO 2 incubator. The results showed that, compared with the control group, immunoglobulin M (IgM) concentration was increased in the supernatant of the 100 μg/ml AAP ‐treated group ( p < .05), and immunoglobulin G (IgG) concentration was increased in the supernatant of the 200 μg/ml of AAP group ( p < .05). In terms of cytokine production, production of interleukin‐1beta ( IL ‐1β), interleukin‐6 ( IL ‐6) and tumour necrosis factor‐alpha ( TNF ‐α) in the supernatant was enhanced in the AAP group in a dose‐dependent function, as well as enhanced mRNA expressions were showed in the cells ( p < .05). The highest concentration of these three cytokines was observed in different AAP groups ( IL ‐1β for 25 μg/ml of AAP , IL ‐6 for 100, and 200 μg/ml of AAP , and TNF ‐α for 100 μg/ml of AAP respectively). The concentration of nitric oxide ( NO ) was increased when using AAP at the concentration of 100 μg/ml ( p < .05) as compared to the control group. No significant effects on inducible nitric oxide synthase, Toll‐like receptor 4 ( TLR 4), myeloid differentiation factor 88 and nuclear factor Kappa B ( NF ‐κB) mRNA level were observed at each concentration of AAP . In conclusion, we found that AAP can specifically promote the production of immunoglobulins (IgM and IgG), cytokines ( IL ‐1β, IL ‐6 and TNF ‐α), as well as the NO concentration in vitro, but not through the activation of the TLR 4/ NF ‐κB signalling pathway.