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The effect of dietary amylose/amylopectin ratio on serum and hepatic lipid content and its molecular mechanisms in growing‐finishing pigs
Author(s) -
Yang C.,
He J.,
Yu B.,
Yu J.,
Mao X. B.,
Chen D. W.,
Yin Y. L.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12884
Subject(s) - amylopectin , amylose , medicine , chemistry , endocrinology , lipid metabolism , ileum , triglyceride , fatty acid , jejunum , gluconeogenesis , propionate , ingestion , metabolism , biology , cholesterol , biochemistry , starch
Summary This study was conducted to investigate the effects of dietary amylose/amylopectin ratio ( DAR ) on serum and hepatic lipid content, luminal short‐chain fatty acid ( SCFA ) concentrations, and the expression of host genes involved in fat and glucose metabolism in liver and mucosa in growing‐finishing pigs. Forty‐eight Duroc × Landrace × Large White pigs (49.8 ± 2.8 kg) were randomly allocated to low amylose/amylopectin ratio ( LR ) and high amylose/amylopectin ratio ( HR ) groups, each group consisting of six replicates (pen) with four pigs per pen. The DAR was 12/88 for LR and 30/70 for HR . Experiment lasted for 67 days. Results showed that, compared with HR group, ingestion of LR significantly increased the liver total lipid and cholesterol concentration ( p < .05) and decreased the serum LDL ‐C concentration ( p < .05). The concentration of propionate, butyrate and total SCFA s in caecum digesta was significantly lower in LR group than in HR group ( p < .05). We observed a significant increase in glucose transporter 2 ( GLUT 2), sodium‐dependent glucose transporter 1 ( SGLT 1) gene expression in LR ‐fed pigs in the jejunum mucosa ( p < .01). A decrease in Na+‐coupled monocarboxylate transporter ( SMCT 1) and free fatty acid receptor 3 ( FFAR 3) expression was found in the ileum mucosa with LR group ( p < .05). Ingestion of LR diet significantly decreased the hexokinase ( p < .01) and tend to decrease the pyruvate kinase ( p = .050) activities in the liver. Meanwhile, the present results indicated that ingestion of LR diet significantly increased the transcription of gluconeogenesis and lipogenic genes such as forkhead box O1 ( FOXO 1), fatty acid synthetase3 ( FAS 3) ( p < .05). These findings demonstrated that high amylopectin has harmful effects on hepatic lipid deposit through the modulation of the liver Foxo1 signalling and should be avoided from one's diet.