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Effect on Rendement Napole genotype on metabolic markers in Ossabaw pigs fed different levels of fat
Author(s) -
Lu H.,
Yan H.,
Ward M. G.,
Stewart T.,
Adeola O.,
Ajuwon K. M.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12720
Subject(s) - genotype , biology , zoology , triglyceride , medicine , endocrinology , cholesterol , gene , biochemistry
Summary We investigated effects of Rendement Napole ( RN ) genotype on metabolic markers in Ossabaw pigs fed diets with different levels of dietary fat. Thirty‐two pigs, belonging to either the wild‐type ( WT , rn + /rn + ) or carrier ( CAR , RN − /rn + ) genotypes ( n = 16/genotype), were divided into two dietary groups, (high fat [ HF ] or low fat [ LF ]) diets, for 12 weeks ( n = 8 pigs/genotype/diet) after which pigs were killed for gene expression analysis by RT ‐ PCR . Feeding HF diet caused increased daily gain ( ADG , p < .05) and final body weight ( BW ) ( p < .05) in comparison with the LF diet ( p < .05). Feed efficiency (gain:feed) was higher ( p < .05) in pigs on the HF and was higher ( p < .05) in CAR pigs compared to WT . There was genotype × diet interaction ( p = .05) on final BW such that CAR animals on LF diet had the same final BW as animals of both genotypes on HF diet. Carrier pigs on LF diet had higher ( p < .05) average daily gain and gain:feed than WT pigs. There was a trend ( p < .08) for a higher feed consumption in pigs on the LF diet. Backfat thickness was higher ( p < .01) in pigs on the HF diet. Serum triglyceride was higher (0.62 vs. 0.33 mg/dl, p < .01) in pigs on HF diet. Serum insulin was higher ( p < .05) in CAR versus WT pigs (0.40 vs. 0.015 μg/ml). Pigs on the HF diet had a higher ( p < .05) serum insulin compared to those on the LF diet (0.032 vs. 0.023 μg/ml). Carnitine palmitoyl transferase 1‐alpha was higher ( p < .05) in the longissimus dorsi and semitendinosus muscles of pigs on HF diet. Acyl‐CoA oxidase I was elevated ( p < .05) in the liver of pigs on HF diet. Fatty acid synthase was lower in the longissimus dorsi muscle, liver and mesenteric fat ( p < .05) of carrier pigs. The RN gene regulates specific metabolic markers in the Ossabaw pigs.