Premium
Mitochondrial biogenesis and PGC ‐1α gene expression in male broilers from ascites‐susceptible and ‐resistant lines
Author(s) -
Khodambashi Emami N.,
Golian A.,
Danesh Mesgaran M.,
Anthony N. B.,
Rhoads D. D.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12706
Subject(s) - mitochondrial biogenesis , biology , mitochondrial dna , gene expression , broiler , mitochondrion , gene , microbiology and biotechnology , andrology , genetics , medicine , endocrinology , zoology
Summary Ascites is a cardiovascular metabolic disease characterized by accumulation of fluid around the heart and in the abdominal cavity that eventually leads to death. This syndrome is the end‐point result of a series of metabolic incidents that are generally caused by impaired oxygen availability. Mitochondria are the major sites of oxygen consumption, therefore major contributors to oxidative stress. Genetic, metabolic and dietary factors can influence variations in mitochondrial biogenesis (mitochondrial size, number and mass) that might have an effect on oxygen consumption and reactive oxygen species production. This study evaluated the effect of genotype on PGC ‐1α mRNA gene expression and mitochondrial biogenesis. These parameters were examined in male broiler chickens at 22 weeks of age from the SUS and RES lines divergently selected for ascites phenotype. From each line, five birds were sampled for right ventricle and breast muscle. Gene expression and mt DNA copy number were assessed by quantitative PCR . Results showed that birds from SUS had significantly higher PGC ‐1α mRNA gene ( p = .033) and mitochondrial DNA copy number ( p = .038) in breast muscle. There was no difference in right ventricle PGC ‐1α expression or mitochondrial DNA copy number between the two lines. These findings indicate that mitochondrial biogenesis and PGC ‐1α mRNA gene expression differ between male broiler chickens from RES and SUS lines in a tissue‐specific manner.