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High non‐esterified fatty acid concentrations promote expression and secretion of fibroblast growth factor 21 in calf hepatocytes cultured in vitro
Author(s) -
Wang J. G.,
Guo Y. Z.,
Kong Y. Z.,
Dai S.,
Zhao B. Y.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12699
Subject(s) - nefa , ketogenesis , medicine , endocrinology , fgf21 , secretion , biology , fatty acid , fibroblast growth factor , chemistry , metabolism , biochemistry , insulin , ketone bodies , receptor
Summary Negative energy balance is considered as the pathological basis of energy metabolic disorders in periparturient dairy cows. Serum non‐esterified fatty acids ( NEFA ) are one of the most important indicators of energy balance status. Fibroblast growth factor 21 ( FGF 21) has been identified as a hepatokine involved in regulation of metabolic adaptations, such as promoting hepatic lipid oxidation and ketogenesis, during energy deprivation. However, the direct effects of NEFA on FGF 21 expression and secretion in bovine hepatocytes are not entirely clear. The objective of this study was to investigate the effects of different NEFA concentrations on FGF 21 expression and secretion in calf hepatocytes cultured in vitro. NEFA were added to the culture solution at final concentrations of 0.6, 1.2, 1.8 and 2.4 mmol/L. After 24 hr of continuous culture, FGF 21 mRNA and protein expression levels in the hepatocytes were determined by real‐time PCR and Western blot respectively. FGF 21 secretion in the supernatant was determined by enzyme‐linked immunosorbent assay ( ELISA ). The results showed that expression and secretion of FGF 21 at 0.6 mmol/L NEFA ‐treated hepatocytes was higher than that of the control group ( p < .05). The FGF 21 expression and secretion were similar at 1.2, 1.8 and 2.4 mmol/L NEFA ‐treated hepatocytes and significantly higher than those observed for controls ( p < .01). These data suggest that high concentrations of NEFA significantly promote FGF 21 expression and secretion in bovine hepatocytes. In particular, this promotion occurs in a dose‐dependent manner and may be involved in the pathological processes of energy metabolism disorders of dairy cows in the peripartum period.