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Involvement of PKA signalling in anti‐inflammatory effects of chitosan oligosaccharides in IPEC ‐J2 porcine epithelial cells
Author(s) -
Yang J. W.,
Tian G.,
Chen D. W.,
Yao Y.,
He J.,
Zheng P.,
Mao X. B.,
Yu J.,
Huang Z. Q.,
Yu B.
Publication year - 2018
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12686
Subject(s) - downregulation and upregulation , inflammation , mannose , tumor necrosis factor alpha , chemistry , receptor , pharmacology , microbiology and biotechnology , biology , biochemistry , endocrinology , immunology , gene
Summary Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti‐inflammatory activity of low‐molecular‐weight chitosan oligosaccharide ( LCOS ) in a porcine small intestinal epithelial cell line ( IPEC ‐J2). The results showed that TNF ‐ α , as inflammation inducer, significantly upregulated the mRNA expression of IL ‐8 and MCP ‐1. Afterwards, LCOS significantly attenuated mRNA expression of IL ‐8 and MCP ‐1 induced by TNF ‐ α in the cells. Mannose ( MAN ), as ligand of mannose receptor, had no effect on the anti‐inflammatory activity of LCOS , which suggested that mannose receptor may not involve in the anti‐inflammatory activity of LCOS in IPEC ‐J2 cells. Interestingly, N‐[2‐(p‐bromocinnamylamino)ethyl]‐5‐isoquinolinesulfonamide 2 HC l hydrate (H89), as PKA (protein kinase A)‐specific inhibitor, reversed the mRNA expression of IL ‐8 when co‐cultured with LCOS . Furthermore, LCOS concentration dependent downregulated the mRNA expression of claudin‐1 compared with TNF ‐ α treatment. However, the trans‐epithelial electric resistance ( TEER ) was not affected by LCOS when co‐cultured with TNF ‐ α in 3 hr. In conclusion, LCOS have a potent anti‐inflammatory activity, and as a feed additives, may be useful for the inhibition of inflammatory process in weaning period of pigs with intestinal inflammation occurring.