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Effect of in ovo injection of threonine on immunoglobulin A gene expression in the intestine of Japanese quail at hatch
Author(s) -
Kermanshahi H.,
Ghofrani Tabari D.,
Khodambashi Emami N.,
Daneshmand A.,
Ibrahim S. A.
Publication year - 2017
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12543
Subject(s) - quail , in ovo , saline , jejunum , incubation , biology , medicine , endocrinology , gene expression , andrology , threonine , zoology , embryo , biochemistry , gene , serine , enzyme , microbiology and biotechnology
Summary The objective of this study was to investigate the effect of in ovo injection of threonine ( THR ) on immunoglobulin A (IgA) gene expression of Japanese quail on hatch day. A total of 540 Japanese quail eggs were assigned into nine groups of 60 each and were set in a single‐stage incubator. Treatments were as follows: non‐injected (control), two diluent levels (0.05 or 0.1 ml saline), two sites of injection (in or under the air sac) and with or without nutrients (0.5 mg/ml THR ). Eggs were injected on d 11 of incubation. On hatch day, after euthanizing hatched quail chicks, the intestine was removed and the jejunum was separated. The relative mRNA expression of jejunal IgA increased (p < 0.05) by the injection of 0.05 ml THR under the air sac when compared to the control group or other treatments of injection. Compared to the control group, no differences were imputable to treatments of 0.1‐ml injections on IgA gene expression. Differences with other injected groups were not significant. It was concluded that injection of 0.05 ml saline containing 0.5 mg THR /ml under the air sac can improve jejunal IgA mRNA expression in newly hatched Japanese quail chicks.