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In vitro gas production of foliage from three browse tree species treated with different dose levels of exogenous fibrolytic enzymes
Author(s) -
López D.,
VázquezArmijo J. F.,
LópezVillalobos N.,
LeeRangel H. A.,
Salem A. Z. M.,
BorquezGastelum J. L.,
DomínguezVara I. A.,
RojoRubio R.
Publication year - 2016
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12467
Subject(s) - incubation , zoology , fermentation , chemistry , food science , botany , in vitro , composition (language) , biology , biochemistry , linguistics , philosophy
Summary The aim of this study was to evaluate the effect of different dose levels of exogenous fibrolytic enzymes ( EFE ) on in vitro ruminal fermentation kinetics and energy utilization of foliages from three browse trees ( Pithecellobium dulce , Heliocarpus velutinus and Guazuma ulmifolia ). Mixture of EFE product was added to the leaves of the three browse tree species at three dose levels: 0 (control), 3.5 and 7.0 mg/g of DM . Chemical composition of the foliages, including plant secondary metabolites such as total phenolics ( TP ), saponins ( SAP ) and aqueous fraction ( AF ), was determined. In addition, in vitro assaying of ruminal gas production kinetics was determined for the three browse three foliages treated with EFE . P. dulce had the highest crude protein content (p < 0.05), whereas G. ulmifolia had the highest content of neutral detergent fibre and SAP (p < 0.05) and H. velutinus had the lowest content of TP (p < 0.05). The interaction between tree species and dose level of EFE was significant (p < 0.05) for gas production ( GP ) at 24 h of incubation, parameters b and c of the accumulated GP curve, short‐chain fatty acids ( SCFA ) and metabolizable energy ( ME ). The lowest (p < 0.01) extent of accumulated GP as well as the b and c values occurred in G. ulmifolia at 0 mg EFE /g DM . P. dulce had the highest (p < 0.05) values for ME and SCFA at the highest dose of EFE . Tree species and dose level had significant (p < 0.05) effects on all parameters describing in vitro ruminal fermentation kinetics and energy utilization. Addition of EFE improved the fermentation kinetics of the browse species considered in this study.