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Feed–drug interaction of orally applied butyrate and phenobarbital on hepatic cytochrome P450 activity in chickens
Author(s) -
Mátis G.,
Kulcsár A.,
Petrilla J.,
HermándyBerencz K.,
Neogrády Zs.
Publication year - 2016
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12416
Subject(s) - cyp3a , butyrate , pharmacology , cytochrome p450 , xenobiotic , sodium butyrate , drug , phenobarbital , drug metabolism , cyp3a4 , chemistry , microsome , pregnane x receptor , biology , biochemistry , enzyme , nuclear receptor , fermentation , gene , transcription factor
Summary The expression of hepatic drug‐metabolizing cytochrome P450 ( CYP ) enzymes may be affected by several nutrition‐derived compounds, such as by the commonly applied feed additive butyrate, possibly leading to feed–drug interactions. The aim of this study was to provide some evidence if butyrate can alter the activity of hepatic CYP s in chickens exposed to CYP ‐inducing xenobiotics, monitoring for the first time the possibility of such interaction. Ross 308 chickens in the grower phase were treated with daily intracoelomal phenobarbital ( PB ) injection (80 mg/kg BW ), applied as a non‐specific CYP ‐inducer, simultaneously with two different doses of intra‐ingluvial sodium butyrate boluses (0.25 and 1.25 g/kg BW ) for 5 days. Activity of CYP 2H and CYP 3A subfamilies was assessed by specific enzyme assays from isolated liver microsomes. According to our results, the lower dose of orally administered butyrate significantly attenuated the PB ‐triggered elevation of both hepatic CYP 2H and CYP 3A activities, which might be in association with the partly common signalling pathways of butyrate and CYP ‐inducing drugs, such as that of PB . Based on these data, butyrate may take part in pharmacoepigenetic interactions with simultaneously applied drugs or other CYP ‐inducing xenobiotics, with possible consequences for food safety and pharmacotherapy. Butyrate was found to be capable to maintain physiological CYP activity by attenuating CYP induction, underlining the safety of butyrate application in poultry nutrition.