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Effects of β ‐hydroxybutyricacid on the synthesis and assembly of very low‐density lipoprotein in bovine hepatocytes in vitro
Author(s) -
Deng Q.,
Ma D.,
Shi Z.,
Huang W.,
Du X.,
Gao W.,
Zhu X.,
Lei L.,
Zhang M.,
Sun G.,
Yuan X.,
Li X.,
Wang Z.,
Liu G.,
Li X.
Publication year - 2016
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12380
Subject(s) - in vitro , chemistry , hepatocyte , low density lipoprotein , lipoprotein , biochemistry , cholesterol
Summary β ‐Hydroxybutyricacid ( BHBA ) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low‐density lipoprotein ( VLDL ) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA . We found that BHBA treatment upregulated the mRNA and protein levels of apolipoprotein B100 (ApoB 100), apolipoprotein E (ApoE) and microsomal triglyceride transfer protein ( MTTP ) and showed in a firstly increased and then decreased trend. Meanwhile, the mRNA and protein levels of LDLR showed in a reverse trend. Consequently, VLDL content was significantly increased in medium‐dose BHBA treatment group, while decreased in high‐dose group. These results indicate that the effects of BHBA on the VLDL synthesis showed in a dose‐dependent manner that low levels of BHBA increase VLDL synthesis and high levels of BHBA decrease VLDL synthesis.