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Antioxidant supplementation of low‐protein diets reduced susceptibility to pulmonary hypertension in broiler chickens raised at high altitude
Author(s) -
Sharifi M. R.,
Khajali F.,
Hassanpour H.
Publication year - 2016
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12333
Subject(s) - broiler , malondialdehyde , uric acid , vitamin e , pulmonary hypertension , antioxidant , zoology , nitric oxide , medicine , feed conversion ratio , chemistry , endocrinology , biology , body weight , biochemistry
Summary A reduced‐protein diet (designated as RPD ) was prepared and its effects on growth performance and the development of pulmonary hypertension syndrome ( PHS ) were evaluated in broiler chickens compared to a normal‐protein diet (designated as NPD ) or to the RPD supplemented with CoQ 10 alone (30 mg/kg) or in combination with vitamin E (30 mg/kg CoQ 10 + 100 mg/kg vitamin E). The RPD had 30 g/kg less crude protein compared to the NPD . A total of 208 1‐day‐old male broilers (Ross 308 strain) were used in a 42‐day trial. Serum concentrations of uric acid ( UA ) and nitric oxide ( NO ) significantly (p < 0.05) declined when chickens fed on the RPD . However, supplementing RPD with the antioxidants significantly (p < 0.05) increased the serum NO concentration. Although serum malondialdehyde ( MDA ) concentration was significantly (p < 0.05) higher in the RPD than the NDP , supplementing RPD with CoQ 10 and CoQ 10 + VE decreased serum MDA concentration to similar levels found in the NPD . Significant overexpression in GPX 1 gene observed in the heart and lungs of broilers fed on the RPD , which was effectively restored by supplementation of CoQ 10 . The right to total ventricular weight ratio ( RV : TV ) was significantly (p < 0.05) increased in birds fed the RPD , which concurred with an increase in mortality from pulmonary hypertension syndrome ( PHS ). However, a significant decline in mortality from PHS was observed when birds on RPD received CoQ 10 or CoQ 10 + VE . In conclusion, antioxidant supplementation effectively improves pulmonary hypertensive response in broiler chicken fed of reduced‐protein diets.