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Effect of L‐lysine on expression of selected genes, serum concentration of amino acids, muscle growth and performance of growing pigs
Author(s) -
Morales A.,
García H.,
Arce N.,
Cota M.,
Zijlstra R. T.,
Araiza B. A.,
Cervantes M.
Publication year - 2015
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12267
Subject(s) - semitendinosus muscle , lysine , loin , amino acid , biology , leucine , zoology , jejunum , myosin , limiting , weight gain , basal (medicine) , body weight , medicine , endocrinology , biochemistry , mechanical engineering , insulin , engineering
Summary Lysine (Lys) is the first limiting amino acid ( AA ) in most feed formulations for pigs and most abundant, along with leucine, in muscle proteins. An experiment was conducted with 17 pigs (17.7 ± 0.05 kg initial BW ) to identify a role of dietary Lys in the control of protein synthesis in pigs. Fourteen pigs were randomly assigned to one of the two wheat‐based dietary treatments: Lys‐deficient, 3.0 g/kg ( DEF ) and Lys‐adequate, 10.8 g/kg ( ADE ). Samples from jejunum mucosa, liver, Longissumus and Semitendinosus muscles, and blood were collected. The other three pigs were sacrificed at the beginning of the trial to measure basal carcass composition. Weight gain, gain:feed ratio, Lys intake and loin eye area were greater in ADE than in DEF pigs (p < 0.01). Muscle‐related carcass characteristics were better, and myosin heavy chain II b expression (My HC II b) in Semitendinosus was higher in ADE than in DEF pigs. Expression of AA transporters CAT ‐1 was lower (p < 0.05), serum Lys was higher and serum Val was lower in pigs fed the ADE diet. The higher muscularity, My HC II b expression in Semitendinosus muscle and Lys serum of pigs fed the ADE diet suggest that Lys increases growth rate not only by functioning as protein construction unit but also as potential control of the protein synthesis process.