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Effects of rare earth element lanthanum on rumen methane and volatile fatty acid production and microbial flora in vitro
Author(s) -
Zhang T. T.,
Zhao G. Y.,
Zheng W. S.,
Niu W. J.,
Wei C.,
Lin S. X.
Publication year - 2015
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12251
Subject(s) - propionate , rumen , lanthanum , dry matter , zoology , chemistry , food science , butyrate , methanogenesis , fatty acid , butyric acid , biology , methane , biochemistry , fermentation , inorganic chemistry , organic chemistry
Summary The objectives of the trial were to study the effects of rare earth element (REE) lanthanum (La) on the in vitro rumen methane (CH 4 ) and volatile fatty acid (VFA) production and the microbial flora of feeds. Four feed mixtures with different levels of neutral detergent fibre (NDF), that is 20.0% (I), 31.0% (II), 41.9% (III) and 52.7% (IV), were formulated as substrates. Five levels of LaCl 3 , that is 0, 0.4, 0.6, 0.8 and 1.0 mmol/kg dry matter (DM), were added to the feed mixtures, respectively, as experimental treatments in a two‐factor 5 × 4 randomized design. The in vitro incubation lasted for 24 h. The results showed that supplementing LaCl 3 increased the total gas (p < 0.001) production and tended to increase the total VFA production (p = 0.072) and decreased the CH 4 production (p = 0.001) and the ratios of acetate/propionate (p = 0.019) and CH 4 /total VFA (p < 0.001). Interactions between LaCl 3 and NDF were significant in total gas production (p = 0.030) and tended to be significant in CH 4 production (p = 0.071). Supplementing LaCl 3 at the level of 0.8 mmol/g DM decreased the relative abundance of methanogens and protozoa in the total bacterial 16S rDNA analysed using the real‐time PCR (p < 0.0001), increased F. succinogenes (p = 0.0003) and decreased R. flavefaciens (p < 0.0001) whereas did not affect R. albus and anaerobic fungi (p > 0.05). It was concluded that LaCl 3 decreased the CH 4 production without negatively affecting feed digestion through manipulating rumen microbial flora when feed mixtures with different levels of NDF were used as substrates.