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The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of W ujin pig
Author(s) -
Liu L. Y.,
Wang J.,
Huang Y.,
Pan H. B.,
Zhang X.,
Huang Z. X.,
Zhao S. M.,
Gao S. Z.
Publication year - 2014
Publication title -
journal of animal physiology and animal nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.651
H-Index - 56
eISSN - 1439-0396
pISSN - 0931-2439
DOI - 10.1111/jpn.12081
Subject(s) - protein biosynthesis , biology , gene expression , messenger rna , blot , rna , medicine , protein expression , gene , muscle protein , phosphorylation , endocrinology , translation (biology) , microbiology and biotechnology , biochemistry , skeletal muscle
Summary The objective of this study was to investigate the regulatory mechanism underlying the increased muscle protein accumulation in pigs while were fed a high protein diet. The eukaryotic initiation factors (e IF s) have been reported to involve in muscle protein synthesis. We investigated the m RNA and protein expression levels of e IF 2 B 1, 4 A 1, 4 B and 4 E in W ujin pigs fed either a high protein ( HP : 18%) or a low protein ( LP : 14%) diet at 30, 60 or 100 kg body weight, based on real‐time PCR and western blotting analyses. Our results indicated that the expression levels of e IF 2 B 1 m RNA and protein were increased by HP diet at all body weight. The HP diet showed higher m RNA and protein levels of e IF 4 B gene at 60 and 100 kg. The protein expression of e IF 4 E phosphorylation was increased by HP diet only at 30 kg. These data suggested that the HP diet promoted porcine muscle protein accumulation mainly by up‐regulating e IF 2 B 1, 4 B and 4 E rather than 4 A 1 expression along the growth stages.