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Melatonin biosynthesis enzymes recruit WRKY transcription factors to regulate melatonin accumulation and transcriptional activity on W‐box in cassava
Author(s) -
Wei Yunxie,
Liu Guoyin,
Chang Yanli,
Lin Daozhe,
Reiter Russel J.,
He Chaozu,
Shi Haitao
Publication year - 2018
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12487
Subject(s) - melatonin , wrky protein domain , enzyme , biosynthesis , transcription (linguistics) , transcription factor , biology , microbiology and biotechnology , biochemistry , medicine , endocrinology , gene , transcriptome , gene expression , linguistics , philosophy
Melatonin is widely involved in growth, development, and stress responses in plants. Although the melatonin synthesis enzymes have been identified in various plants, their interacting proteins remain unknown. Herein, overexpression of tryptophan decarboxylase 2 (Me TDC 2)‐interacting proteins, N ‐acetylserotonin O ‐methyltransferase 2 (Me ASMT 2) interacting proteins, and N ‐acetylserotonin O ‐methyltransferase 3 (Me ASMT 3) in cassava leaf protoplasts resulted in more melatonin than when other enzymes were overexpressed. Through yeast two‐hybrid, 14 Me TDC 2‐interacting proteins, 24 Me ASMT 2 interacting proteins, and 9 Me ASMT 3‐interacting proteins were identified. Notably, we highlighted Me WRKY 20 and Me WRKY 75 as common interacting proteins of the 3 enzymes, as evidenced by yeast two‐hybrid, and in vivo bimolecular fluorescence complementation (Bi FC ). Moreover, co‐overexpression of Me TDC 2/Me ASMT 2/3 with Me WRKY 20/75 in cassava leaf protoplasts did not only activated the transcriptional activities of Me WRKY 20 and Me WRKY 75 on W‐box, but also induced the effects of Me TDC 2, Me ASMT 2/3 on endogenous melatonin levels. Taken together, 3 melatonin synthesis enzymes (Me TDC 2, Me ASMT 2/3) interact with Me WRKY 20/75 to form a protein complex in cassava. This information significantly extends the knowledge of the complex modulation of plant melatonin signaling.