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Melatonin reverses H 2 O 2 ‐induced senescence in SH ‐ SY 5Y cells by enhancing autophagy via sirtuin 1 deacetylation of the RelA/p65 subunit of NF ‐κB
Author(s) -
Nopparat Chutikorn,
Sinjanakhom Puritat,
Govitrapong Piyarat
Publication year - 2017
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12407
Subject(s) - melatonin , autophagy , sirtuin 1 , acetylation , microbiology and biotechnology , senescence , protein subunit , sirtuin , biology , homeostasis , chemistry , endocrinology , biochemistry , downregulation and upregulation , apoptosis , gene
Autophagy, a degradation mechanism that plays a major role in maintaining cellular homeostasis and diminishes in aging, is considered an aging characteristic. Melatonin is an important hormone that plays a wide range of physiological functions, including the anti‐aging effect, potentially via the regulation of the Sirtuin1 ( SIRT 1) pathway. The deacetylation ability of SIRT 1 is important for controlling the function of several transcription factors, including nuclear factor kappa B ( NF ‐ ĸB ). Apart from inflammation, NF ‐ ĸB can regulate autophagy by inhibiting Beclin1, an initiator of autophagy. Although numerous studies have revealed the role of melatonin in regulating autophagy, very limited experiments have shown that melatonin can increase autophagic activity via SIRT 1 in a senescent model. This study focuses on the effect of melatonin on autophagy via the deacetylation activity of SIRT 1 on RelA/p65, a subunit of NF ‐ ĸB , to determine whether melatonin can attenuate the aging condition. SH ‐ SY 5Y cells were treated with H 2 O 2 to induce the senescent state. These results demonstrated that melatonin reduced a number of beta‐galactosidase ( SA ‐βgal)‐positive cells, a senescent marker. In addition, melatonin increased the protein levels of SIRT 1, Beclin1, and LC 3‐ II , a hallmark protein of autophagy, and reduced the levels of acetylated‐Lys310 in the p65 subunit of NF ‐ ĸB in SH ‐ SY 5Y cells treated with H 2 O 2 . Furthermore, in the presence of SIRT 1 inhibitor, melatonin failed to increase autophagic markers. The present data indicate that melatonin enhances autophagic activity via the SIRT 1 signaling pathway. Taken together, we propose that in modulating autophagy, melatonin may provide a therapeutically beneficial role in the anti‐aging processes.