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Melatonin regulates lipid metabolism in porcine oocytes
Author(s) -
Jin JunXue,
Lee Sanghoon,
Taweechaipaisankul Anukul,
Kim Geon A.,
Lee Byeong Chun
Publication year - 2017
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12388
Subject(s) - melatonin , lipogenesis , lipolysis , lipid metabolism , biology , mitochondrial biogenesis , perilipin , lipid droplet , endocrinology , medicine , tfam , downregulation and upregulation , mitochondrion , microbiology and biotechnology , biochemistry , adipose tissue , gene
It is being increasingly recognized that the processes of lipogenesis and lipolysis are important for providing an essential energy source during oocyte maturation and embryo development. Recent studies demonstrated that melatonin has a role in lipid metabolism regulation, including lipogenesis, lipolysis, and mitochondrial biogenesis. In this study, we attempted to investigate the effects of melatonin on lipid metabolism during porcine oocyte in vitro maturation. Melatonin treatment significantly enhanced the number of lipid droplets ( LD s) and upregulated gene expression related to lipogenesis ( ACACA , FASN , PPAR γ, and SREBF 1). Oocytes treated with melatonin formed smaller LD s and abundantly expressed several genes associated with lipolysis, including ATGL , CGI ‐58, HSL , and PLIN 2. Moreover, melatonin significantly increased the content of fatty acids, mitochondria, and ATP , as indicated by fluorescent staining. Concomitantly, melatonin treatment upregulated gene expression related to fatty acid β‐oxidation ( CPT 1a, CPT 1b, CPT 2, and ACADS ) and mitochondrial biogenesis ( PGC ‐1α, TFAM , and PRDX 2). Overall, melatonin treatment not only altered both the morphology and amount of LD s, but also increased the content of fatty acids, mitochondria, and ATP . In addition, melatonin upregulated mRNA expression levels of lipogenesis, lipolysis, β‐oxidation, and mitochondrial biogenesis‐related genes in porcine oocytes. These results indicated that melatonin promoted lipid metabolism and thereby provided an essential energy source for oocyte maturation and subsequent embryonic development.