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Identification, transcriptional and functional analysis of heat‐shock protein 90s in banana ( M usa acuminata L .) highlight their novel role in melatonin‐mediated plant response to F usarium wilt
Author(s) -
Wei Yunxie,
Hu Wei,
Wang Qiannan,
Zeng Hongqiu,
Li Xiaolin,
Yan Yu,
Reiter Russel J.,
He Chaozu,
Shi Haitao
Publication year - 2017
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12367
Subject(s) - melatonin , biology , fusarium wilt , fusarium oxysporum , heat shock protein , wilt disease , pathogen , auxin , hsp90 , abiotic component , microbiology and biotechnology , heat shock , botany , horticulture , gene , genetics , ecology , neuroscience
As one popular fresh fruit, banana ( Musa acuminata ) is cultivated in the world's subtropical and tropical areas. In recent years, pathogen Fusarium oxysporum f. sp. cubense ( Foc ) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. However, the molecular mechanism of banana response to Foc remains unclear, and functional identification of disease‐related genes is also very limited. In this study, nine 90 kDa heat‐shock proteins ( HSP 90s) were genomewide identified. Moreover, the expression profile of them in different organs, developmental stages, and in response to abiotic and fungal pathogen Foc were systematically analyzed. Notably, we found that the transcripts of 9 MaHSP90s were commonly regulated by melatonin ( N ‐acetyl‐5‐methoxytryptamine) and Foc infection. Further studies showed that exogenous application of melatonin improved banana resistance to Fusarium wilt, but the effect was lost when cotreated with HSP 90 inhibitor (geldanamycin, GDA ). Moreover, melatonin and GDA had opposite effect on auxin level in response to Foc4 , while melatonin and GDA cotreated plants had no significant effect, suggesting the involvement of Ma HSP 90s in the cross talk of melatonin and auxin in response to fungal infection. Taken together, this study demonstrated that Ma HSP 90s are essential for melatonin‐mediated plant response to Fusarium wilt, which extends our understanding the putative roles of Ma HSP 90s as well as melatonin in the biological control of banana Fusarium wilt.