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2‐Hydroxymelatonin promotes the resistance of rice plant to multiple simultaneous abiotic stresses (combined cold and drought)
Author(s) -
Lee HyeJung,
Back Kyoungwhan
Publication year - 2016
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12347
Subject(s) - abiotic component , ohm , proline , abiotic stress , transporter , biology , osmoprotectant , amino acid , botany , chemistry , biochemistry , gene , ecology , physics , quantum mechanics
We investigated the physiological roles of 2‐hydroxymelatonin (2‐ OHM el) in rice seedlings. When they were challenged with simultaneous multiple abiotic stressors, such as a combination of cold and drought, those pretreated with 2‐ OHM el were resistant, whereas no tolerance was observed in seedlings treated with either melatonin or water (control). The tolerance phenotype was associated with the induction of several transporter proteins, including the proton transporter ( UCP 1 ), potassium transporter ( HKT 1 ), and water channel protein ( PIP 2;1 ). Treatment with 2‐ OHM el increased the content of the osmoprotectant proline and maintained mitochondrial structure when plants were subjected to a combination of cold and drought stress. We screened the corresponding transcription factors ( TF s) for 2‐ OHM el‐mediated resistance to the combined stressors through analysis of large numbers of cold‐ and drought‐related TF s. Two TF s, Myb4 and AP 37 , were only induced by 2‐ OHM el treatment. Transgenic rice lines overexpressing rice Myb4 were not resistant to the combined stressors; however, the expression of UCP 1 , HKT 1 , and PIP 2;1 transcripts was slightly enhanced. These data show that 2‐ OHM el alleviates the effects of simultaneous abiotic stressors via the actions of multiple TF s, including Myb4 and AP 37 .