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Melatonin induces class A1 heat‐shock factors ( HSFA 1s) and their possible involvement of thermotolerance in Arabidopsis
Author(s) -
Shi Haitao,
Tan DunXian,
Reiter Russel J.,
Ye Tiantian,
Yang Fan,
Chan Zhulong
Publication year - 2015
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12219
Subject(s) - arabidopsis , melatonin , heat shock protein , biology , heat shock , microbiology and biotechnology , endogeny , abiotic stress , hsp90 , heat shock factor , gene , mutant , hsp70 , genetics , biochemistry , endocrinology
Melatonin (N‐acetyl‐5‐methoxytryptamine) serves as an important signal molecule during plant developmental processes and multiple abiotic stress responses. However, the involvement of melatonin in thermotolerance and the underlying molecular mechanism in Arabidopsis were largely unknown. In this study, we found that the endogenous melatonin level in Arabidopsis leaves was significantly induced by heat stress treatment, and exogenous melatonin treatment conferred improved thermotolerance in Arabidopsis . The transcript levels of class A1 heat‐shock factors ( HSFA 1 s), which serve as the master regulators of heat stress responses, were significantly upregulated by heat stress and exogenous melatonin treatment in Arabidopsis . Notably, exogenous melatonin‐enhanced thermotolerance was largely alleviated in HSFA 1s quadruple knockout ( QK ) mutants, and HSFA 1s ‐activated transcripts of heat‐responsive genes ( HSFA 2 , heat stress‐associated 32 ( HSA 32 ), heat‐shock protein 90 ( HSP 90 ), and 101 ( HSP 101 )) might be contributed to melatonin‐mediated thermotolerance. Taken together, this study provided direct link between melatonin and thermotolerance and indicated the involvement of HSFA 1s ‐activated heat‐responsive genes in melatonin‐mediated thermotolerance in Arabidopsis .