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Pinealectomy interferes with the circadian clock genes expression in white adipose tissue
Author(s) -
Farias Talita da Silva Mendes,
Oliveira Ariclécio Cunha,
Andreotti Sandra,
Amaral Fernanda Gaspar,
Chimin Patrícia,
Proença André Ricardo Alves,
Torres Leal Francisco Leonardo,
Sertié Rogério Antonio Laurato,
Campana Amanda Baron,
Lopes Andressa Bolsoni,
Souza Arnaldo Henrique,
CipollaNeto José,
Lima Fabio Bessa
Publication year - 2015
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12211
Subject(s) - endocrinology , medicine , pinealectomy , melatonin , biology , circadian rhythm , clock , adipose tissue , pineal gland , white adipose tissue , circadian clock , leptin , gene expression , hormone , gene , biochemistry , obesity
Abstract Melatonin, the main hormone produced by the pineal gland, is secreted in a circadian manner (24‐hr period), and its oscillation influences several circadian biological rhythms, such as the regulation of clock genes expression (chronobiotic effect) and the modulation of several endocrine functions in peripheral tissues. Assuming that the circadian synchronization of clock genes can play a role in the regulation of energy metabolism and it is influenced by melatonin, our study was designed to assess possible alterations as a consequence of melatonin absence on the circadian expression of clock genes in the epididymal adipose tissue of male W istar rats and the possible metabolic repercussions to this tissue. Our data show that pinealectomy indeed has impacts on molecular events: it abolishes the daily pattern of the expression of C lock, P er2, and C ry1 clock genes and P parγ expression, significantly increases the amplitude of daily expression of Rev‐erbα, and affects the pattern of and impairs adipokine production, leading to a decrease in leptin levels. However, regarding some metabolic aspects of adipocyte functions, such as its ability to synthesize triacylglycerols from glucose along 24 hr, was not compromised by pinealectomy, although the daily profile of the lipogenic enzymes expression ( ATP ‐citrate lyase, malic enzyme, fatty acid synthase, and glucose‐6‐phosphate dehydrogenase) was abolished in pinealectomized animals.