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Melatonin influences proliferation and differentiation of rat dental papilla cells in vitro and dentine formation in vivo by altering mitochondrial activity
Author(s) -
Liu Jie,
Zhou Hongyu,
Fan Wenguo,
Dong Weiguo,
Fu Shenli,
He Hongwen,
Huang Fang
Publication year - 2013
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/jpi.12002
Subject(s) - melatonin , in vivo , in vitro , alkaline phosphatase , microbiology and biotechnology , medicine , endocrinology , chemistry , biology , biochemistry , enzyme
Abstract Melatonin mediates a variety of biological processes ranging from the control of circadian rhythms to immune regulation. Melatonin also influences bone formation and osteointegration of dental implants. However, the effects of melatonin on dentine formation have not been examined. This study investigated the effects of melatonin on the proliferation and differentiation of rat dental papilla cells ( r DPC s) in vitro and dentine formation in vivo. We found that melatonin (0, 10 −12 , 10 −10 ,10 −8 m ) induced a dose‐dependent reduction in r DPC s proliferation, increased alkaline phosphatase ( ALP ) activity, the expression of dentine sialoprotein ( DSP ), and mineralized matrix formation in vitro. In vivo melatonin (50 mg/kg, BW , i.p.) inhibited dentine formation. Melatonin (10 −8 m ) suppressed the activity of complex I and IV in the basal medium ( OS −) and enhanced the activity of complex I and complex IV in osteogenic medium ( OS +). These results demonstrate that melatonin suppresses the proliferation and promotes differentiation of r DPC s, the mechanisms of which may be related to activity of mitochondrial complex I and complex IV .