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Reinigung und teilweise Charakterisierung von Papaya Ringspot Virus
Author(s) -
DE LA ROSA MERCEDES,
LASTRA R.
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.1983.106.4.329
Subject(s) - biology , capsid , papaya ringspot virus , virus , nucleic acid , virology , strain (injury) , peg ratio , plant virus , density gradient , rna , biochemistry , potyvirus , gene , physics , finance , anatomy , quantum mechanics , economics
A high incidence of papaya ringspot virus (PRSV) was found during a survey of viruses affecting papaya in Venezuela. The Venezuelan strain has a thermal inactivation point between 52 and 54° C, dilution end point of 10 −4 , and the longevity in vitro was 24 h. The modal length of 167 virus particles was 782 nm. The bouyant density of purified virus particles in CsCl was 1.323 g/cm 3 . The virus nucleic acid was identified as a single stranded RNA molecule. The molecular weight of the virus capsid protein was 28,000 daltons. Purification was carried out by chloroform — carbon tetrachloride, followed by precipitation with 6 % PEG. The virus was further purified in CsCl density gradient.