Premium
Reinigung und teilweise Charakterisierung von Papaya Ringspot Virus
Author(s) -
DE LA ROSA MERCEDES,
LASTRA R.
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.1983.106.4.329
Subject(s) - biology , capsid , papaya ringspot virus , virus , nucleic acid , virology , strain (injury) , peg ratio , plant virus , density gradient , rna , biochemistry , potyvirus , gene , physics , finance , anatomy , quantum mechanics , economics
A high incidence of papaya ringspot virus (PRSV) was found during a survey of viruses affecting papaya in Venezuela. The Venezuelan strain has a thermal inactivation point between 52 and 54° C, dilution end point of 10 −4 , and the longevity in vitro was 24 h. The modal length of 167 virus particles was 782 nm. The bouyant density of purified virus particles in CsCl was 1.323 g/cm 3 . The virus nucleic acid was identified as a single stranded RNA molecule. The molecular weight of the virus capsid protein was 28,000 daltons. Purification was carried out by chloroform — carbon tetrachloride, followed by precipitation with 6 % PEG. The virus was further purified in CsCl density gradient.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom