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Über einen antiviralen Faktor in Chenopodium amaranticolor ‐Pflanzen infiziert mit dem Tabaknekrosevirus: Extraktion, Reinigung, chemische und biologische Eigenschaften
Author(s) -
FACCIOLI G.,
CAPPONI R.
Publication year - 1983
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.1983.106.4.289
Subject(s) - biology , chenopodium , tobacco mosaic virus , inoculation , pronase , plant virus , glycoprotein , microbiology and biotechnology , biochemistry , virus , virology , botany , enzyme , horticulture , trypsin , weed
In leaves of Chenopodium amaranticolor locally infected by Tobacco necrosis virus (TNV), 3 days after inoculation an inhibitor of virus biosynthesis was present. Such antiviral factor (AVF) has been extracted, cleared with hydrated calcium phosphate and partially purified by DEAE‐column chromathography. AVF was also present in upper uninoculated leaves of plants inoculated on the lower leaves which acquired a partial systemic resistance against a TNV‐challenge inoculation. AVF binds to Sepharose‐Concavalin‐A and is partially recovered from it by elution buffer containing 4 % α ‐methyl‐D‐glucoside. AVF looses its antiviral activity when treated with alkaline phosphate, α ‐glucosidase and pronase (under conditions suitable for proteolysis of glycoproteins), while it does not when treated with snake venom phosphodiesterase, β ‐glucosidase, lipase and DNase. It seems, therefore to be a phosphorylated glycoprotein. Its production is inhibited by actinomycin D, and this lead to think that its formation depends on the same mechanism which is responsible for the synthesis of cellular RNA, DNA‐dependent.