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Molecular characterization of phytoplasma diseases of pepper in Turkey
Author(s) -
Yilmaz Salih,
Caglar Behcet Kemal,
Djelouah Khaled
Publication year - 2019
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12820
Subject(s) - biology , phytoplasma , phylogenetic tree , restriction fragment length polymorphism , pepper , horticulture , botany , sequence analysis , polymerase chain reaction , gene , genetics
During autumn, an extensive survey was conducted in pepper ( Capsicum annum L.) in intensive cultivation areas of four provinces in southeastern Turkey (Adana, Kahramanmaraş, Mersin and Şanlıurfa) in order to identify the causal agent (s) of phytoplasma‐like symptoms (chlorosis, little‐leaf, short internodes and stunting). DNA amplification by PCR and RFLP analysis using EcoRI restriction enzyme confirmed the presence of phytoplasmas in Şanlıurfa and Mersin, and consequently their possible association with the symptoms. Sequencing and phylogenetic analysis revealed that the isolate from Şanlıurfa had 99% sequence identity with “ Candidatus Phytoplasma trifolii” (16SrVI) and is a member of the clover proliferation group (16SrVI‐A). Additionally, the isolate from Mersin had 96% sequence identity with “ Candidatus Phytoplasma asteris” (16SrI). Importantly, gene sequence of the Mersin isolate shared <97.5% similarity to previously discovered “ Ca . Phytoplasma” species. Consequently, the phytoplasma detected from Mersin could represent a new “ Ca. Phytoplasma” species and to our knowledge, this is the first report of asteris‐like phytoplasmas infecting pepper in Turkey.