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Candidatus Phytoplasma trifolii (16Sr VI ) infection modifies the polyphenols concentration in pepper ( Capsicum annuum ) plant tissues
Author(s) -
RevelesTorres Luis Roberto,
VelásquezValle Rodolfo,
SalasMuñoz Silvia,
MauricioCastillo Jorge Armando,
EsquedaDávila Karla Caren Jacqueline,
Herrera Mayra Denise
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12717
Subject(s) - phytoplasma , biology , pepper , restriction fragment length polymorphism , botany , polyphenol , horticulture , 16s ribosomal rna , nested polymerase chain reaction , polymerase chain reaction , bacteria , gene , genetics , biochemistry , antioxidant
Of late, the presence of Candidatus Phytoplasma trifolii was reported as a serious threat to the pepper crop in Zacatecas, México; therefore, asymptomatic and symptomatic pepper plants were collected from a commercial field among three samplings after the fruit set stage was reached. Total DNA was extracted using the CTAB ‐based method and tested for phytoplasma using a nested PCR assay, followed by a BLAST , and restriction fragment length polymorphism ( RFLP ) analysis of 16S rDNA sequences, which confirmed the presence of phytoplasma group 16Sr VI , “ Candidatus Phytoplasma trifolii” in the symptomatic plants. As the metabolic pathways of pathogen‐infected plants tend to change, resulting in a biochemical differentiation with the noninfected plants, the polyphenolic compound concentrations were quantified from the vegetative tissues (root, stem, leaves and developed fruit/big bud) and were analysed based on a principal component analysis ( PCA ). Results revealed that, in general, plants tend to a progressive increase in total phenols, flavonoids, condensed tannins and anthocyanins related to the plants exposure to “ Ca . P. trifolii” infection, and PCA demonstrated that almost 90% of the observed variance was explained by the first two components. Hence, the phenolic content of the plants increases as a response of the defence mechanism, which reflects its condition and resistance.