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Isolation and identification of Sydowia polyspora and its pathogenicity on Pinus yunnanensis in Southwestern China
Author(s) -
Pan Yue,
Ye Hui,
Lu Jun,
Chen Peng,
Zhou XuDong,
Qiao Min,
Yu ZeFen
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12696
Subject(s) - pinus yunnanensis , biology , botany , bark (sound) , phloem , inoculation , endophyte , fungus , bark beetle , horticulture , ecology
Abstract Sydowia polyspora occurs often as an epiphyte or endophyte of conifers and is widely distributed around the world. It is also reported as a fungus associated with bark beetles. In this study, based on ITS sequence data and morphological characteristics, 14 strains collected from the beetle bodies and galleries of Tomicus minor and Tomicus yunnanensis infesting Pinus yunnanensis in Southwestern China were identified as S. polyspora . To assess the virulence of S. polyspora to P. yunnanensis , pathogenicity tests were conducted by inoculating the fungus in the stems and on needles of P. yunnanensis . The results after 30 days showed that the lesion lengths in the phloem after inoculation with S. polyspora were significantly longer than for the control. In addition, the fungus caused partly distinct chlorisis or discolouration of needles 14 days after inoculation. This suggests that S. polyspora is able to colonize stem phloem and pine needles of P. yunnanensis and thus may cause secondary damages to this host after being spread with bark beetles.