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Resistance to Phytophthora pathogens is dependent on gene silencing pathways in plants
Author(s) -
Guo Na,
Zhao Jinming,
Yan Qiang,
Huang Jing,
Ma Huifei,
Rajput Nasir Ahmed,
Jiang Hua,
Xing Han,
Dou Daolong
Publication year - 2018
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/jph.12695
Subject(s) - biology , nicotiana benthamiana , gene silencing , rna silencing , phytophthora , rna induced silencing complex , trans acting sirna , rna induced transcriptional silencing , phytophthora sojae , genetics , gene , rna interference , rna , botany
RNA silencing is one of the main defence mechanisms employed by plants to fight pathogens. p19 protein encoded by the tomato bushy stunt virus ( TBSV p19) is known as a suppressor of RNA silencing via si RNA sequestration to prevent the assembly of RISC . To better understand the impact of TBSV p19 on silencing and its roles in Phytophthora pathogens, we used the transient expression assay in Nicotiana benthamiana and found that the leaves expressing TBSV p19 were more susceptible to Phytophthora parasitica . Furthermore, we demonstrated that TBSV p19‐mediated plant susceptibility in N. benthamiana is dependent on RNA ‐dependent RNA polymerase 6 ( RDR 6). We also tested the role of RNA silencing in resistance of soybean hairy roots to Phytophthora . The lesion size induced by P. sojae on TBSV p19‐expressing soybean hairy roots was slightly, but significantly larger than GFP ‐expressing soybean hairy roots. Finally, the Arabidopsis gene silencing mutants ago1‐27 , zip‐1 , sgs3‐11 and rdr6‐11 were also examined for their resistance to P. parasitica . The results clearly showed that resistance levels of the mutants were visibly reduced compared with the wild type. Taken together, these results suggest that the gene silencing system in plants is essential for resistance to Phytophthora pathogens.